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cts n 2 supplement
Cts N 2 Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n-+2+supplement/Casein/pmc12796728-36-0-4
Average 96 stars, based on 1 article reviews
Cts N 2 Supplement, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/n-+2+supplement/Casein/pmc12796728-36-0-4
Average 96 stars, based on 1 article reviews
cts n 2 supplement - by Bioz Stars,
2026-10
96/100 stars
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Recombinant:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% BIA-KA:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% SYBR Green Assay:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Labeling:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Cloning:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Mutagenesis:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Software:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Protease Inhibitor:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Magnetic Beads:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Milk:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Membrane:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Western Blot:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Enzyme-linked Immunosorbent Assay:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Blocking Assay:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Derivative Assay:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Migration:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Cell Culture:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% Co-Culture Assay:Article Title: GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection. Article Snippet: 2.11 | AKP cells co- culture with H. pylori AKP cells were cultured in collagen type I solution from rat tail (Sigma) pre- coated T flasks (Falcon) in Minigut medium composed of DMEM/ F12 (Gibco), 1% Penicillin/streptomycin, 1% Article Title: Modulation of human dorsal root ganglion neuron firing by the Nav1.8 inhibitor suzetrigine. Article Snippet: Each well was then gently flooded with 1 mL of culture media consisting of BrainPhys media (Stemcell technologies, Cat. 05709), 1% penicillin/streptomycin, 1% GlutaMAX, 2% NeuroCult SM1 (Stemcell technologies, Cat 05711), 1% Article Title: Pharmacologically inducing regenerative cardiac cells by small molecule drugs Article Snippet: When the CMs recovered contraction, the culture medium was changed with chemically reprogramming medium (MCDB131 Gibco), 25 mg/l NaHCO3 (Sigma), 4 mM Glucose (Sigma), 1% Article Title: Variant-specific effects of GBA1 mutations on dopaminergic neuron proteostasis. Article Snippet: Then, the media was changed to N2B27 neuronal cell culture medium composed of 49% Article Title: High levels of chromosomal instability facilitate the tumor growth and sphere formation. Article Snippet: The HeLa Kyoto cell line is a popular subline of HeLa cells, a human cervical carcinoma- derived cell line, which is suitable for mitotic study due to its slow migration.24 In spheroid culture, cells were seeded to round- bottomed ultra- low attachment 96- well plates (Corning) at 1000 cells/well in renal epithelial cell growth basal medium (REBM) without FBS, and supplemented with Article Title: Assembly of neuron- and radial glial-cell-derived extracellular matrix molecules promotes radial migration of developing cortical neurons Article Snippet: DOI: https://doi.org/10.7554/eLife.92342 21 of 32 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Peptide, recombinant protein Recombinant Human Neurocan Protein R&D 6508- NC- 050 Peptide, recombinant protein Chondroitinase ABC Protease Free Seikagaku Corporation 100332 Commercial assay or kit BCA assay kit Thermo Fisher 23227 Commercial assay or kit RNeasy Mini Kit Qiagen 74104 Commercial assay or kit PowerUp SYBR Green Master Mix Thermo Fisher A25741 Commercial assay or kit DIG RNA Labeling Kit (SP6/T7) Roche 11175025910 Commercial assay or kit NEBuilder HiFi DNA Assembly Master Mix New England Biolabs E2621 Commercial assay or kit In- Fusion HD Cloning Kit Takara 639648 Commercial assay or kit KOD -Plus- Mutagenesis Kit Toyobo SMK- 101 Software, algorithm MASCOT server Matrix science https://www. matrixscience.com/server. html RRID:SCR_014322 Software, algorithm ImageJ Schneider et al., 2012 https://imagej.nih.gov/ij/ Other DAPI solution DOJINDO Lot.PF082 340–07971 Other N- 2 Supplement Thermo Fisher 17502–048 Other B- 27 Supplement Thermo Fisher 17504–044 Other Neurobasal Medium Thermo Fisher 21103–049 Other Penicillin- Streptomycin Thermo Fisher 15070–063 Other Article Title: Electrically Conductive Injectable Silk/PEDOT: PSS Hydrogel for Enhanced Neural Network Formation. Article Snippet: The majority of cell culture reagents including neurobasal medium, DMEM/F- 12, fetal bovine serum (FBS), N- 2 supplement, MEM nonessential amino acids (NEAA), β- mercaptoethanol, B- 27 supplement, GlutaMAX, and Article Title: A synthetic method to assay polycystin channel biophysics Article Snippet: Cells were centrifuged at 300 × g for 4 min and plated on polylysine- coated glass coverslip at 1–4 × 105 density in basal medium eagle solution containing B27 supplement (17504044, Gibco), N- 2 supplement (17502048, Gibco), 0.5% |